a2780 (human ovarian cancer cells, cl-0013)  (Procell Inc)

Journal: Nature Communications

Article Title: Mitochondrial segmentation and function prediction in live-cell images with deep learning

doi: 10.1038/s41467-025-55825-x

Figure Lengend Snippet: a An illustrative scheme presents the distinct mitochondrial morphological trends of drug-sensitive and -resistant human ovarian cancer cells upon cisplatin treatment (created with BioRender.com). b MoDL-generated pseudo-color images (1024 × 1024 pixel 2 resolution) display the mitochondrial morphology under cisplatin treatment (0, 1, 5, 10 μM) for 24 h, and the corresponding morphological quantitative features ( c ), including mean area, form factor, branch length, and solidity. The blue and orange dot represent A2780 S and A2780 CP cells, respectively. n = 85 images (512 × 512 pixel 2 resolution), scale bars = 2 μm. d The impact of collecting diverse sample sizes from a pre-existing dataset on the MSE in MoDL’s predictions (initial training set = 1120, test set = 320, i.e., 70 and 20 images in 2048 × 2048 pixel 2 resolution, respectively). e MoDL’s predictions of changes in MMP polarization, ATP generation, ROS production, mitophagy level, and respiration rate in A2780 S (blue line) and A2780 CP (orange line) cells treated with cisplatin (0, 1, 5, 10 μM) for 24 h. The dash and solid lines represent prediction and ground truth (biochemical assays), respectively. n = 3 independent experiments. Data are given as the mean ± SD ( c , e ). The original experimental biochemical results are provided in Supplementary Data ( e ) Statistical differences were calculated using a two-way ANOVA, A2780 S vs. A2780 CP cells and cisplatin treatment 10 μM groups vs . control ( c ) or a two-sided Mann–Whitney test, prediction vs . ground truth ( e ). No significant (ns, P > 0.05). Source data are provided as a file.

Article Snippet: The cell lines L02 (human normal liver cell, CL-0111) and A2780 (human ovarian cancer cells, CL-0013) were purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Generated, Control, MANN-WHITNEY

Journal: Journal of Ovarian Research

Article Title: Shikonin reduces M2 macrophage population in ovarian cancer by repressing exosome production and the exosomal galectin 3-mediated β-catenin activation

doi: 10.1186/s13048-024-01430-3

Figure Lengend Snippet: SK treatment suppresses malignant phenotype of OC cell lines. A , IC50 value of SK to SKOV3 and A2780 cells tested by applying different doses of SK (1 µM, 2 µM, 4 µM, 8 µM, 16 µM, 32 µM, 64 µM, 128 µM, and 256 µM) for 48 h, examined by CCK-8 assay. SKOV3 and A2780 cells were treated with 5 µM SK for 48 h. B , colony formation capacity of SKOV3 and A2780 cells determined by colony formation assay; C , migration ability of cells analyzed by wound healing assay; D , invasion ability of cells determined by Transwell assay; E , apoptosis of cells measured by TUNEL assay. Three biological replicates were performed. Differences were analyzed by two-way ANOVA. * p < 0.05

Article Snippet: OC cell lines SKOV3 (CL-0215) and A2780 (CL-0013), and a human monocytic leukemia cell line THP-1 (CL-0233), were acquired from Procell Life Science & Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques: CCK-8 Assay, Colony Assay, Migration, Wound Healing Assay, Transwell Assay, TUNEL Assay

Journal: Journal of Ovarian Research

Article Title: Shikonin reduces M2 macrophage population in ovarian cancer by repressing exosome production and the exosomal galectin 3-mediated β-catenin activation

doi: 10.1186/s13048-024-01430-3

Figure Lengend Snippet: SK treatment reduces the promoting effect of OC cell-derived exo on M2 polarization of macrophages. Exo from SKOV3 and A2780 cells, either with or without 5 µM SK treatment for 48 h, were collected, which were designated to OC exo and SK OC exo, respectively. A , morphology of isolated OC exo or SK OC exo determined under TEM; B , particle size distribution and concentration of isolated OC exo or SK OC exo examined by NTA; C , protein expression of exo marker proteins CD9, CD63 and CD81 and endoplasmic reticulum marker Calnexin in the isolated OC exo or SK OC exo determined by WB analysis; D , successful uptake of DiO-labeled exo by THP-1 cells observed under the fluorescence microscope. THP-1 cells were stimulated with 150 nM PMA for 24 h to differentiate into M0 macrophages, followed by treatment with PBS, OC exo or SK OC exo. E - F , percentage of CD163- ( E ) and CD206-( F ) positive THP-1 cells examined by flow cytometry; G , production of IL-10 in the culture supernatant of THP-1 cells examined using ELISA; H , mRNA expression of CD163 and CD206 in THP-1 cells determined by RT-qPCR. Three biological replicates were performed. Differences were analyzed by the one-way or two-way ANOVA. * p < 0.05

Article Snippet: OC cell lines SKOV3 (CL-0215) and A2780 (CL-0013), and a human monocytic leukemia cell line THP-1 (CL-0233), were acquired from Procell Life Science & Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques: Derivative Assay, Isolation, Concentration Assay, Expressing, Marker, Labeling, Fluorescence, Microscopy, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Extracellular vesicles of Bifidobacterium longum reverse the acquired carboplatin resistance in ovarian cancer cells via p53 phosphorylation on Ser15

doi: 10.1002/kjm2.12837

Figure Lengend Snippet: Effect of Bifidobacterium longum ‐EVs on the proliferation, apoptosis, migration, and invasion of A2780 cells. A2780 cells were incubated with 1, 5, and 10 μg/mL of B. longum ‐EVs (defined as B. longum ‐EVs‐L, B. longum ‐EVs‐M, and B. longum ‐EVs‐H groups, respectively). (A) Cell viability was measured by the Cell Counting Kit‐8 (CCK‐8) after treatment for 24, 48, and 72 h. After a 24 h treatment, (B) cell proliferation, (C) apoptosis, (D) migration, and (E) invasion were detected by Edu staining, Annexin V‐FITC/PI double staining, wound healing, and Transwell assays, respectively. ** P < 0.01 and *** P < 0.001, versus the control group.

Article Snippet: The A2780 OVC cell line (Procell no. CL‐0013) was grown in DMEM media with 10% FBS at 37°C.

Techniques: Migration, Incubation, Cell Counting, CCK-8 Assay, Staining, Double Staining, Control

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Extracellular vesicles of Bifidobacterium longum reverse the acquired carboplatin resistance in ovarian cancer cells via p53 phosphorylation on Ser15

doi: 10.1002/kjm2.12837

Figure Lengend Snippet: Bifidobacterium longum ‐EVs enhances the sensitivity of A2780‐CBP/R cells to carboplatin (CBP). A2780‐CBP/R cells from different groups were treated with 10 μM of CBP, 1 μg/mL of B. longum ‐EVs, or the combination, whereas cells treated with PBS were considered the control. (A) The cell viability was detected by CCK‐8 assay after treatment for 24, 48, and 72 h. After a 24‐h treatment, (B) cell proliferation, (C) apoptosis, (D) migration, and (E) invasion were detected by Edu staining, Annexin V‐FITC/PI double staining, wound healing, and Transwell assays, respectively. * P < 0.05, ** P < 0.01, and *** P < 0.001, versus the control group; # # P < 0.01, and ### P < 0.001, versus the CBP group.

Article Snippet: The A2780 OVC cell line (Procell no. CL‐0013) was grown in DMEM media with 10% FBS at 37°C.

Techniques: Control, CCK-8 Assay, Migration, Staining, Double Staining

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Extracellular vesicles of Bifidobacterium longum reverse the acquired carboplatin resistance in ovarian cancer cells via p53 phosphorylation on Ser15

doi: 10.1002/kjm2.12837

Figure Lengend Snippet: Bifidobacterium longum ‐EVs promote p53 Ser15 phosphorylation to increase p53 accumulation in A2780‐CBP/R cells. (A) A2780‐CBP/R cells from different groups were treated with 10 μM carboplatin (CBP), 1 μg/mL of B. longum ‐EVs, or the combination, whereas the cells treated with PBS were considered the control. After treatment for 24 h, cells from each group were harvested to analyze the expression levels of MRP1, ATP7A, ATP7B, and p53. (B) Extracts from A2780‐CBP/R cells from the B. longum ‐EVs‐L, B. longum ‐EVs‐M, and B. longum ‐EVs‐H groups were immunoprecipitated with either control IgG or p53 antibodies, and the presence of p53 Ser15, Ser20, and Ser46 phosphorylation was determined by western blot analysis. (C) A2780‐CBP/R cells were transfected with p53S15D or p53wt or the combination with or without B. longum ‐EVs. The cells were then treated with MG132 for an additional 6 h before collecting cell lysates for the ubiquitination assay. *** P < 0.001, versus the control group; # # P < 0.01, and ### P < 0.001, versus the CBP group.

Article Snippet: The A2780 OVC cell line (Procell no. CL‐0013) was grown in DMEM media with 10% FBS at 37°C.

Techniques: Phospho-proteomics, Control, Expressing, Immunoprecipitation, Western Blot, Transfection, Ubiquitin Proteomics

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Extracellular vesicles of Bifidobacterium longum reverse the acquired carboplatin resistance in ovarian cancer cells via p53 phosphorylation on Ser15

doi: 10.1002/kjm2.12837

Figure Lengend Snippet: Bifidobacterium longum ‐EVs reverse carboplatin (CBP) resistance by promoting p53 Ser15 phosphorylation in A2780‐CBP/R cells. The p53S15D mutant was generated in A2780‐CBP/R cells. A2780‐CBP/R cells with the p53S15A mutant were treated with CBP alone or combined with B. longum‐ EVs, whereas the cells treated with PBS served as the control group. (A) Cell viability was measured by the CCK‐8 assay after treatment for 24, 48, and 72 h. After a 24‐h treatment, (B) cell proliferation, (C) apoptosis, (D) migration, and (E) invasion were detected by Edu staining, Annexin V‐FITC/PI double staining, wound healing, and Transwell assays, respectively. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The A2780 OVC cell line (Procell no. CL‐0013) was grown in DMEM media with 10% FBS at 37°C.

Techniques: Phospho-proteomics, Mutagenesis, Generated, Control, CCK-8 Assay, Migration, Staining, Double Staining